Steinernema neocurtillae

Nguyen & Smart, 1992

DESCRIPTION

Males, first generation: (FIG.1)Body enlarged and curved posteriorly, C-shaped when heat-killed. Head rounded, labial papillae not seen in side view. Stoma very shallow to almost absent, usually with a pronounced sclerotized structure at anterior end. Distance from anterior end to excretory pore extremely short, less than body width at excretory pore. Esophagus almost cylindrical anteriorly, isthmus indistinct, basal bulb slightly swollen. Gonad monorchic, reflexed. About 60% of males with normal testis, 40% with reduced or collapsed testes. In males with normal testis, distance from base of esophagus to anterior end of testis always shorter than distance from anterior end of nematode to base of esophagus. Spicules paired, brown in color. Head (manubrium) of spicules elongate (FIG.SEM) , in some, twice as long as wide, and about one third the spicule length; shaft (calomus) very short or absent; blade (lamina) thick, tapering slightly posteriorly, about twice as long as head; blade terminus blunt with a depression on ventral side; head/blade angle 132 degrees (120-141); velum rarely seen. Each spicule with 2 internal ribs. Gubernaculum long, boat-shaped, anterior part long, ventrally curved, more than three fourths spicule length; gubernaculum length divided by spicule length 0.89 (0.82-0.93). Eleven pairs and one single precloacal genital papillae distributed as in. Tail conoid; tail terminus of 70% of males with short mucron.

 Males, second generation:. Second-generation male similar to that of the first generation except smaller, thinner, and distance from head to reflexion of testis much longer. Collapsed testis, found in first generation, very rare in second generation. Spicules and gubernaculum shorter and thinner than those of first generation male. Spicule with head/blade angle 151 degrees (143-160) (N=10). Gubernaculum almost as long as spicules, gubernaculum length divided by spicule length (G/S) 0.95 (0.92-1.0) (in other species G/S 0.52-0.75). Mucron on tail terminus very rare.

 Females, first generation:(FIG.2) Body cuticle smooth or with faint annules. Lateral fields and phasmids not observed. Head rounded, continuous with body; perioral disc present; labial papillae 6, obscure, not seen in side view (seen clearly in side view of other species), 4 cephalic papillae. Lips indistinct. Amphids not observed. Stoma shallow, posterior part of stoma subtriangular. In lateral view, cheilorhabdions prominent, well sclerotized; another smaller sclerotization posterior to cheilorhabdions, presumably the prorhabdions. Esophagus with procorpus cylindrical, muscular; metacorpus slightly swollen; isthmus indistinct; basal bulb slightly enlarged. Nerve ring surrounds isthmus, usually obscure. Pronounced esophago-intestinal valve present. Excretory pore well anterior; distance from anterior end to excretory pore usually shorter than body width at excretory pore. Excretory cell very large. Gonads amphidelphic, reflexed, often containing many eggs. Vulva, a transverse slit, protruding on females with juveniles in body but not protruding on young females; double-flapped epiptygma present. Vagina sclerotized, short, about one-fourth body width, perpendicular to body in mature females, but directed posteriorly in young females. Body width greater anterior to vulva than posterior to vulva. Tail shape variable, ventral postanal swelling present or absent, tail usually shorter than anal body width. About 25% of population with tail longer than anal body width.

 Females, giant form of first generation: In addition to the normal females of the first generation, a giant form of the female occurs along with regular first-generation females. The giant form is characterized by a twisted body and is two to three times longer than normal females of the first generation (10,313 - 13,000 micrometers compared to 3,531 - 6,250 micrometers). Other structures are as described for normal females.

 Females, second generation: Similar to first generation female but smaller, and median bulb of esophagus slightly more enlarged. Ovaries containing at most three rows of eggs laterally. Tail, which tapers to a point, longer than anal body width; ventral postanal swelling present.

 Infective juveniles(IJ): Body thin, elongate. Sheath (second-stage cuticle) present but sometimes lost. Labial region continuous. Oral aperture narrow Under electron microscope, labial papillae not seen; 4 cephalic papillae prominent (6 labial and 4 cephalic papillae prominent in S. carpocapsae); amphids present. Distance from anterior end to excretory pore extremely short, averaging 18 m vs. 38-83 m in other species. D% (distance from anterior end to excretory pore divided by esophagus length) and E% (distance from anterior end to excretory pore divided by tail length) 12 and 23, respectively. Esophagus with thin anterior part, basal bulb more or less elongate with visible valve. Lateral field with 7 incisures forming 6 bands, sometimes 8 incisures (7 bands) were observed. Tail attenuate, tapering gradually.

TYPE HOST AND LOCALITY

The nematode Steinernema neocurtillae was found in hemocoel of northern mole cricket, Neocurtilla hexadactylla collected near La Crosse (Alachua County), Florida.

DISTRIBUTION AND HOSTS

Steinernema neocurtillae has been found in Florida, USA and possibly all over the country where the nematode s host, Northern mole cricket Neocurtilla hexadactilla was reported. So far, this mole cricket has been the only host for the nematode.

BIONOMICS AND HOST PARASITE RELATIONSHIPS

It was difficult to produce the nematode in vivo in the lab. IJ's placed in soil in petri dishes infected and killed Neocurtilla hexadactylla but few IJs were produced in the cadavers. Infected mole crickets collected from the field produced large numbers of IJ's.

BACTERIAL ASSOCIATE

No bacteria were reported from Steinernema neocurtillae.

BIOCONTROL CAPABILITY

No information is available on the use of this nematode in biological control of insects.
 
Table 1. Morphometric characters
Character1 Infective juvenile (50) 1st gen female (40) 1st gen male (10)
Body length 885 SD 44 (741-988) 8049 SD 860 (3531-13000) 1593 SD 231 (1283-1988)
Width 34 SD 2.7 (28-42) 218 SD 16 (159-289) 111 SD 20 (77-144)
Stoma length - 11 SD 1.6 (6-17) 4.7 SD 0.8 (4-6)
Stoma width - 13 SD 2.2 (8-20) 6.9 SD 2 (3-9)
EP 18 SD 1.7 (14-22) 41 SD 8 (23-59) 31 SD 5 (19-34)
NR 107 SD 4 (100-119) 216 SD 25 (153-295) 123 SD 15 (108-158)
ES 144 SD 7 (130-159) 287 SD 30 (181-356) 165 SD 29 (125-231)
Testis flexure 360 SD 90 (279-512)
Tail 80 SD 5 (64-97) 61 SD 9.5 (47-78) 25 SD 6 (19-39)
ABW 70 SD 12 (50-100) 41 SD 4 (39-50)
Mucron length 4 SD 0.8 (3-5)
Spicule length (SP) 59 SD 4 (52-64)
Spicule width 12 SD 1.7 (9-14)
Gubern.length (GU) 52 SD 4 (44-59)
Gubern. width 8 SD 1 (6-9)
Vulva% 50 SD 3 (42-56)
a 26 SD 2 (22-29)
b 6 SD 0.3 (5-7)
c 11 SD 0.8 (9-14)
D% 12 SD 1 (10-15) 15 SD 3 (8-20) 19 SD 4 (13-26)
E% 23 SD 2 (18-30)
SW= SP/ABW 1.43 SD 0.14 (1.18-1.64)
GS=GU/SP 0.89 SD 0.04 (0.81-0.93)

1 EP = Distance from anterior end to excretory pore; NR = Distance from anterior end to nerve ring; ES = Distance from anterior end to end of esophagus; ABW = anal body width; a = body length/greatest body width; b = body length/ES; c = body length/ tail length; D%=EP/ES x 100; E%=EP/tail length x 100.

LITERATURE CITED

Nguyen, K. B., G. C. Smart, Jr. (1992). Steinernema neocurtillis n. sp. (Rhabditida: Steinernematidae) and a key to species of the genus Steinernema. Journal of Nematology


This document was constructed and is maintained by KHUONG B. NGUYEN
Entomology & Nematology Department
University of Florida