A description is given of Steinernema anatoliense  (Rhabditida: Steinemematidae), an entomopathogenic nematode isolated from soil samples collected in the locality of Kars, East Anatolia, Turkey. Cross hybridisation, morphological and molecular studies (RFLP analysis) indicated the distinctness of S. anatoliense  from other Steinernema species. Diagnostic characters include: body length of third stage infective juvenile (507 5801um); lateral field pattern with 6, 8 longitudinal ridges; tail shape of the first generation male with a cuticular mucro; shape of the spicules and gubemaculum; and arrangement of the genital papillae. In addition, RFLP analysis of the ITS region of rDNA shows S. anatoliense  to be distinct from 50 other Steinernema species and isolates.

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