Steinernema
robustispiculum
Abstract Steinernemar robustispiculum n. sp. (Rhabditida:
Steinernematidae) was isolated from woodland in Chumomray National Park, Sason,
Sathay, Kontum, Vietnam. Its morphology, morphometrics, cross-hybridisation and
the ITS-rDNA sequence analysis revealed that S. robustispiculum clearly
differs from other known Steinernema spp. As in the cases of S.
intermedium (Poinar, 1985), S. robustispiculum has very robust
spicules, but it can be distinguished by the longer tail of the infective
juvenile, lower E%, shorter spicules, the shape of the spicules, the number of
genital papillae in the caudal region and the presence of a mucron on the male
tail. S. robustispiculum has a lateral field resembling that of S.
sangi Phan, Nguyen & Moens, 2001, but can be distinguished by a higher
E%, higher D%, smaller length to width ratio of the spicules and the morphology
of both the spicule head (manubrium) and the dorsal lobe of the spicule. The
morphometrics of infective juveniles of S. robustispiculum are similar
to those of S. monticolum Stock, Choo & Kaya, 1997; these species
can be distingusihed by the position of the excretory pore, the smaller length
to width ratio of the spicules, and the length and morphology of the spicule
head (manubrium). The phylogenetic relationships within Steinernema
Travassos, 1927, including the newly sequenced Vietnamese species S.
robustispiculum n. sp., S. loci Phan, Nguyen & Moens, 2001, S.
thanhi Phan, Nguyen & Moens, 2001 and S. sangi, are presented
based on analyses of the ITS-rDNA. The ITS RFLP profiles obtained from 17
different restriction enzymes are also presented.
(More details will be added
later)
Reference:
Long K. Phan, Sergei A. Subbotin,
Lieven Waeyenberge and Maurice Moens (2005). A new
entomopathogenic nematode, Steinernema robustispiculum n. sp.
(Rhabditida: Steinernematidae), from Chumomray National Park in Vietnam.
Systematic Parasitology 60:23-32
1 Institute of Ecology and Biological Resources, National Centre for Science
and Technology, Hoang Quoc Viet 18, Nghiado, Caugiay, Hanoi, Vietnam
2 Institute of Parasitology of Russian Academy of Sciences, Leninskii
Prospect 33, Moscow, 117071, Russia
3 CLO-Department for Crop Protection, Burg. Van Gansberghelaan 96,
Merelbeke, B 9820, Belgium
4 Laboratory for Agrozoology, Gent University, Coupure 555, Gent, 9000,
Belgium.